Journal: EMBO Reports

Article Title: Developmental epigenetic programming by Tet1/3 determines peripheral CD8 T cell fate

doi: 10.1038/s44319-025-00439-z

Figure Lengend Snippet: ( A ) Absolute numbers of mature CD8 + T cells from control Tet1/3fl/fl and Rorc(t)CreTgTet1/3fl/fl mice. (WT = 5, Tet1/3 cDKO = 8 biological replicates). Data are mean ± SEM, unpaired t test. ( B ) Representative FACS plots of CD62L and CD44 expression on CD8 T cells and frequency of naive CD8 T cells from indicated secondary lymphoid organs of control P14 Tet1/3fl/fl and P14-Rorc(t)CreTgTet1/3fl/fl mice, pregated on LiveTCRb+CD8a+ cells. (WT = 3, Tet1/3 cDKO = 3 biological replicates). Data are mean ± SEM, multiple unpaired t tests. ( C ) Relative expression of Tet2 normalized to HPRT in naive Tet1/3fl/fl and Rorc(t)CreTgTet1/3fl/fl CD8 T cells. (WT = 3, Tet1/3 cDKO = 2 biological replicates). ( D ) Representative FACS plots and quantification of frequency and number of GP276- and NP396-tetramer+ CD8 T cells. (WT = 3-4, Tet1/3 cDKO = 5 biological replicates) Data are mean ± SEM, multiple unpaired t tests. ( E ) Representative FACS plots and quantification of frequency and number of SLEC and MPECs among GP276-tetramer+ and NP396-tetramer+ CD8 cells. (WT = 3-4, Tet1/3 cDKO = 5 biological replicates) Data are mean ± SEM, multiple unpaired t tests. ( F ) RT-qPCR assessment of LCMV copy number (CN) per spleen 5dpi. (WT = 3, Tet1/3 cDKO = 3 biological replicates) Data are mean ± SEM, unpaired t test. ( G ) Absolute number of GP33-tetramer+ CD8 T cells 24dpi. (WT = 4, Tet1/3 cDKO = 6 biological replicates). Data are mean ± SEM, unpaired t test. ( H ) Representative FACS and quantification of frequency and number of TEM/TCM 8dpi. (WT = 10, Tet1/3 cDKO = 9 biological replicates). Data are mean ± SEM, multiple unpaired t tests. Data information: P values are indicated in the figures and P < 0.05 was considered significant; paired male or female mice were used and no gender biases associated with genotypes were observed. ( A , B , H ) Experiments were replicated at least three times.

Article Snippet: vF500 anti-mouse CD44 (clone IM7) , Tonbo , Cat#: 85-0441.

Techniques: Control, Expressing, Quantitative RT-PCR

Journal: Cancer Cell International

Article Title: CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin

doi: 10.1186/s12935-020-01663-4

Figure Lengend Snippet: CD44 cross-linking in breast cancer. a Expression of CD44 in breast cancer cell lines MCF-7, T-47D, BT-549 and MDA-MB-231. On the left, qRT-PCR results of CD44,on the right, western blot analysis of CD44; b Expression of CD44 cross-linking in breast cancer cell lines; c , d Comparison of migration and invasion in breast cancer cell lines

Article Snippet: Primary antibodies against CD44 (Abcam, ab119348), CD44 (clone IM7, Cat #14-0441-86), ERM (CST, 3142S), p-ERM (CST, 3141S), Moesin (Abcam, ab52490) p-Moesin (Abcam, ab177943), p-Merlin(CST, 13281S), Merlin (CST, 12888) were used.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Comparison, Migration

Journal: Cancer Cell International

Article Title: CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin

doi: 10.1186/s12935-020-01663-4

Figure Lengend Snippet: CD44 cross-linking is associated with the malignancy of breast cancer. a The effects of different concentrations of hyaluronidase on CD44 cross-linking of BT-549 and MDA-MB-231 cells were detected by Western Blot assay. b , c The effect of 300 μg/ml hyaluronidase on the cell migration and invasion ability of BT-549 and MDA-MB-231 cells; *P < 0.05, ***P < 0.001

Article Snippet: Primary antibodies against CD44 (Abcam, ab119348), CD44 (clone IM7, Cat #14-0441-86), ERM (CST, 3142S), p-ERM (CST, 3141S), Moesin (Abcam, ab52490) p-Moesin (Abcam, ab177943), p-Merlin(CST, 13281S), Merlin (CST, 12888) were used.

Techniques: Western Blot, Migration

Journal: Cancer Cell International

Article Title: CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin

doi: 10.1186/s12935-020-01663-4

Figure Lengend Snippet: Moesin phosphorylation can be affected by CD44 cross-linking in breast cancer cells. a Detection of Moesin expression in breast cancer cell lines by qRT-PCR; b Detection of ERM, p-ERM and Merlin expression in breast cancer cell lines by western blot; c Effects of hyaluronidase at different concentrations on the expression of ERM and Merlin and their phosphorylated forms; d p-Moesin and ERM expression after CD44 antibody mediated cross-linking in MDA-MB-231 cells

Article Snippet: Primary antibodies against CD44 (Abcam, ab119348), CD44 (clone IM7, Cat #14-0441-86), ERM (CST, 3142S), p-ERM (CST, 3141S), Moesin (Abcam, ab52490) p-Moesin (Abcam, ab177943), p-Merlin(CST, 13281S), Merlin (CST, 12888) were used.

Techniques: Phospho-proteomics, Expressing, Quantitative RT-PCR, Western Blot

Journal: Cancer Cell International

Article Title: CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin

doi: 10.1186/s12935-020-01663-4

Figure Lengend Snippet: Moesin shRNA knockdown attenuated the promotion of CD44 cross-linking on cell metastasis and invasion. a p-Moesin and Moesin expression of BT-549 and MDA-MB-231 and Moesin knockdown cells after treatment with hyaluronidase. b , c Migration of BT-549 and MDA-MB-231 and Moesin knockdown cells after treatment with hyaluronidase; d , e Invasion of BT-549 and MDA-MB-231 and Moesin knockdown cells after treatment with hyaluronidase; ***P < 0.001, NS no significance

Article Snippet: Primary antibodies against CD44 (Abcam, ab119348), CD44 (clone IM7, Cat #14-0441-86), ERM (CST, 3142S), p-ERM (CST, 3141S), Moesin (Abcam, ab52490) p-Moesin (Abcam, ab177943), p-Merlin(CST, 13281S), Merlin (CST, 12888) were used.

Techniques: shRNA, Knockdown, Expressing, Migration